3. The rate and direction of ion movement is
governed by the electrochemical gradient.
The rate of ion transport through the channel is
very high 107 ions/sec.
Transport is always down the gradient.
3
4. In the late1800s, the chemical mechanism
underlying nerve and muscle tissue messaging
was not known.
Ludimar Hermann was able to conclude that nerve
and muscle cells were capable of exhibiting a "self-
propagating wave of negative charge which
advances in steps along the tissue ".
4
5. In 1880s, Sidney Ringer used a solution of water and
ran it through the vessels of an isolated heart from
a frog and discovered that in order for the heart to
continue beating salts needed to be present in the
water.
Sodium, calcium, and potassium salts were
important and had to be in specific concentration
relative to each other.
5
6. In 1970s, the existence of ion channels was
confirmed by the invention of ‘patch clamp’
technique by Erwin Neher and Bert Sakmann who
won a Nobel Prize for it.
In 2003, the Nobel Prize was awarded to American
scientists, Roderick MacKinon and Peter Agre for
their x-ray crystallographic structure studies on
ion channels.
6
7. Permit ions of a specific size and charge.
The permeating ions will lose their
dissociated water molecules and pass
through the hole in the channel which is
known as SELECTIVITY FILTER.
This limits their rate of passage.
7
8. Two discrete states – open(conducting) or
closed(nonconducting)
8
9. change in the voltage
across the membrane
(voltage-gated channels)
the binding of a ligand
(ligand-gated
channels)
9
Confirmational Changes
13. Voltage Gated ion channels are made of three basic
parts:
1) The transmembrane pore
2) Voltage sensor
3) Selectivity filter
Contains different
subunits:
α subunit and other auxillary
Subunits.
13
17. These channels are responsible for the rapid
influx of sodium ions during the action
potential in nerve, muscle, and endocrine
cells.
17
18. 18
VOLTAGE GATED ION CHANNELS
ION CHANNELS AGONIST ANTAGONIST
V.G. SODIUM
CHANNEL
Aconitine Local anaesthetics,
Type 1 anti-
arrythmic drugs
19. Voltage-gated calcium channels mediate calcium
influx in response to membrane depolarization and
regulate intracellular processes such as contraction,
secretion, neurotransmission.
There are several different kinds of voltage-gated
calcium channels:
o L-type: skeletal, smooth, cardiac muscle
(contraction) and for aldosterone secretion
in endocrine cells of the adrenal cortex.
19
20. o N-type: Pre synaptic terminal and involved in
neurotransmission.
o T-type : neurons, bones(osteocytes). Control the
pace-making activity of the SA Node within the
heart.
o R-type: neurons, dentrites. Control of firing
pattern.
o P/Q-type channel: Purkinje neurons in the
cerebellum.
20
21. 21
ION CHANNELS AGONIST ANTAGONIST
CALCIUM
CHANNEL
L Bay K8644 Dihydropyridines,
verapamil, diltiazem
N ____ ω conotoxin,
gabapentin,
lamotrigin,
levetiracetam
T ____ Mibefradil
P/Q ___ ω agatoxin
22. Voltage-gated K+ channels are one of the key
components in generation and propagation of electrical
impulses in nervous system. Upon changes in
transmembrane potential, these channels open and
allow passive flow of K+ ions from the cell to restore the
membrane potential.
Agonist: Retigabine; Antagonist: 4-amino pyridine,
TEA.
22
These channels are present in every type
of neuron, where they control excitability, restore
the resting membrane potential and help regulate
cell volume.
24. 24
TRANSMEMBRANE PROTEIN which
open to allow ions such as Na+, K+,
Ca2+, or Cl− to pass through the
membrane in response to the
binding of a chemical messenger (i.e.
a ligand).
They are all receptors.
33. 1) Ion channel History:
http://www2.montana.edu/cftr/ionchannelprimers/i
on_channel_history2.htm
2) Rang and Dale, seventh edition
3) K D Tripathi
4) Glutamate and glutamate receptors in the vertebrate
retina by Victoria Connaughton
5) Molecular Cell Biology, Sixth Edition
6) Helen C. Lai and Lily Y. Jan; The distribution and
targeting of neuronal voltage-gated ion channels;
Nature Reviews Neuroscience 7, 548-562 (July
2006)
7) Beverly hills; Scholarpedia; Ion Channels
8) Ligand gated ion channels:
http://lenoverelab.org/LGICdb/LGICdb.php
9) http://www.britannica.com/science/ion-channel
10) http://www.snipview.com/q/Ion%20channel
33