Joining Discovery on Target 2016 in Boston, for Targeting the Microbiome, DrBonnie presents new discoveries in research, technology, and upcoming companies. Most importantly, DrBonnie360 focuses on the gut as an organ of immunity and its possible relations to autoimmune disease. Topics include: Brain-Gut Axis, Locality, The Leaky Gut, Microbial Composition, Dysbiosis, Oral Microbiome, Personalizing Nutrition, Crowdscience, and Medicines from the Microbiome. Company Summary Tables: Information in order of Indication, What, and How.

1. Trust Your Gut
Second Annual Targeting the Microbiome September 20-21, 2016 Bonnie Feldman DDS, MBA @DrBonnie360
Your Autoimmunity Connection
Data and Digital Tools for the Microbiome:
An Emerging Field in Autoimmune Disease

2. Trust Your Gut
+1.310.666.5312
drbonnie360@gmail.com
www.drbonnie360.com
@DrBonnie360
www.facebook.com/YourAutoimmunityConnection
Bonnie Feldman, DDS, MBA
DrBonnie360’s mission is to create a digitally
connected world of personalized care for
autoimmune patients.
We consult with start-up companies and
entrepreneurs who are bringing new products and
services that will improve research, diagnosis, and
treatment for autoimmunity

3. The Road to Today

4. 2008
2012
2015
2016
The NIH Human Microbiome
Project is established
Public Curiosity Ignites
Scientific American publishes
an interactive human
microbiome infographic
Microbiome exhibit opens to
the public
The Secret World Inside You
The White House announces the
National Microbiome Intiative
Advances in research begin to
show connections to autoimmunity
@DrBonnie360

5. An Invisible Epidemic
@DrBonnie360
~50 Million Americans have an autoimmune disease
DOUBLED
< 6% of NIH Funding goes to Autoimmunity
Source: http://bit.ly/1m0ttsl http://bit.ly/1B57pNb
incidence rate in children
under 10 with IBD
in other words,
More than Cancer + Heart
Disease combined
1 in 6 Americans
Population of 25 states!

6. Is Your Gut
Friend or Foe?

7. The Gut
is Where
It’s At
Brain-Gut Axis
DOI: 10.1038/nrn3346 @DrBonnie360

8. DOI: 10.1038/ajgsup.2012.4 @DrBonnie360
Location
Matters

9. Changing with Age
DOI: 10.1038/nm.4142 @DrBonnie360

10. Oral Diseases
26 APR 2016 DOI: 10.1111/odi.12472
http://onlinelibrary.wiley.com/doi/10.1111/odi.12472/full#odi12472-fig-0002
CLINICAL
PRESENTATION
@DrBonnie360
Triggering Autoimmunity

11. DOI:10.1038/ni.2611 @DrBonnie360
When
Things
Go Wrong
The Leaky Gut

12. The Leaky
Gut in
Celiac
Disease
DOI: 10.3390/nu7085314

13. DOI:10.1038/nrgastro.2015.34 @DrBonnie360
A Life
Balance
Inflammatory
Bowel Disease

14. DOI:10.1038/ajgsup.2012.4 @DrBonnie360
Diversity
Matters
Inflammatory
Bowel Disease

15. DOI:10.1038/nrrheum.2011.121 @DrBonnie360
Beyond the
Gut
Dysbiosis May
Affect Other
Areas
Rheumatoid
Arthritis

16. Beyond the
Gut
Dysbiosis
&
Rheumatoid
Arthritis
DOI:10.1038/nm.3916 @DrBonnie360

17. Beyond the
Gut
Dysbiosis
&
Lupus
@DrBonnie360 DOI:10.1128/mBio.01548-14

18. Beyond the Gut Microbe Composition & Multiple Sclerosis
@DrBonnie360 DOI: 10.1038/ncomms12015

19. DOI:10.1016/j.imlet.2014.08.017 @DrBonnie360
The Oral
Microbiome
Dental Caries
&
Periodontal
Disease

20. Caring for your Microbiome
Your Personal Microbial Garden

21. A
Tailor-Made
Personalizing
Nutrition
DOI:10.111/nyas.13118 @DrBonnie360

22. DOI:10.1016/j.cell.2015.11.001 @DrBonnie360
Tailor-Made
Personalizing
Nutrition

23. Company IndicaKon What How
Open Biome
TherapeuKc - Oral &
Fecal Transplant
Nonprofit Stool Bank & Research
Pla5orm eliminates pracKcal
barriers to Fecal Microbiota
Transplants (FMT) & provide
tailored fecal microbiota
preparaKons to bioinformaKcs/
analyKcal services
-Donor SelecKon; characterize with
molecular biomarkers, cross-secKonal data,
& staKsKcal tools to predict which donors
are well suited.
-Maintenance Therapy; oral capsules to
deliver & explore long-term management of
chronic diseases
uBiome Crowdsourced Database Equip individuals with the tools
needed to learn about bacteria
within their bodies
-Send sample kits & surveys to collect
microbiomes for analysis.
-Use NIH human microbiome project to
sequence DNA & ID each strain
-Compare with library & compile results
Tailor-Made Global Crowdscience for the Microbiome

24. DOI:10.1038/nbt.2548 @DrBonnie360
Tailor-
Made
Medicines
From
Microbiota

25. Company IndicaKon What How
AOBiome TherapeuKc - Topical
Skin
Advance the science of
Ammonia Oxidizing Bacteria
(AOB) to restore the skin’s
microbiome
-Topical therapeuKc & consumer
product of AOB to reintroduce
Nitrosomonas to skin’s bacterial flora &
restore Nitric Oxide balance
Enterome DiagnosKcs &
TherapeuKc
Inflammatory Bowel
Disease - Crohn’s &
UlceraKve ColiKs
QuanDtaDve Metagenomics
Pla5orm (QMP) & FuncDonal
Metagenomics Pla5orm
(FMP) to support
personalized medicine for
microbiome-related diseases
-QMP uses total fecal bacterial gene to
characterize personal metagenome
with disease phenotype & ID
biomarkers
-FMP screen genomic/metagenomic
libraries for dis & dev of new drugs/
targets
Miomics Bio-
TherapeuKcs
TherapeuKc - Oral
Autoimmune
Disease - RA & MS
Oral biologicals to rebalance
a microbiota towards a
standard microbiota.
-Research GI inhabitan commensal

26. Company IndicaKon What How
Osel TherapeuKc - Topical
Women’s Health
“Live BiotherapeuDc
Products” (LBP) focusing on
women’s health (GU & GI)
-Library of proprietary human bacterial
strains
-Screening methods to ID strains w/
unique protecKve properKes
-Produce stable high quality LBP from
selected strains
Second Genome TherapeuKc - Oral
Crohn’s Disease
Microbiome ModulaDng
Discovery Pla5orm (MMDP)
ID key microbial community
members/acKviKes/
metabolites & assay for
modulatory potenKal against
host targets
-Measure & ID changes in the microbial
community composiKon/funcKon/
signaling in health or diseased states w/
MMDP
-GeneraKon & eval of small molecules/
pepKde biologic/bacterial strains that
modulate microbe-microbe & microbe-
human interacKons
Seres TherapeuKcs TherapeuKc - Topical
Dysbiosis, UlceraKve
ColiKs
Propritary Microbiome
TherapeuDcs Pla5orm
(PMTP) & EcobioDc drugs to
reestablish a healthy
microbiome
-ComputaKonal approaches & PMTP to
ID organismal/funcKonal networks of
microbial communiKes, & determine
which organisms work together best
with disease microbiome to restore
funcKon

27. Company IndicaKon What How
Synlogic TherapeuKc
Rare GeneKc
Metabolic Disorders
& IBD
SyntheDc BioDcs engineered
probioKc bacteria
-Plahorm that engineers new metabolic
funcKon into the microbiome
Vedanta Biosciences TherapeuKc
Autoimmune
Modulate pathways of
interacKon between human
microbiome & host immune
sys. To induce tolerance &
restore intesKnal
homeostasis
-Study mucosal immunology to
produce drug based on a proprietary
consorKa of potent regulatory T cell-
inducing Clostridium bacteria
Whole Biome
DiagnosKcs &
TherapeuKc
Metabolic
Syndrome
Proprietary discovery
pla5orm for targeted
equilibraKon of the
microbiome. Focus on
metabolic syndrome
-Target novel symbioKc funcKonality
inherent in the human-microbial
interface with a microbiome diagnosKc
discovery plahorm

28. • Autoimmune Disease: An Invisible Epidemic
http://bit.ly/1m0ttsl
• The Serious Health Concern All 20-
somethings Should Know About
http://bit.ly/R9cEgp
• Decoding Autoimmunity – a Summary of the
State of the Art
http://bit.ly/1knzaAQ
• The Lonely Voices of Autoimmune Disease via
Youtube
http://bit.ly/X41mMv
• Believe It or Not – The Real Voices of
Autoimmunity
http://bit.ly/20xRCXO
Your Autoimmunity Exploration

29. Exploring The Microbiome Lifeline
• Fasano, Alessio. “Leaky Gut and Autoimmune Diseases.” Clinical Reviews in Allergy & Immunology 42.1
(2012) : 71–8. <http://www.ncbi.nlm.nih.gov/pubmed/22109896>.
• Gomez, Andres, David Luckey, and Veena Taneja. “The Gut Microbiome in Autoimmunity: Sex
Matters.”Clinical Immunology 159.2 (2015) : 154–162. <
http://www.sciencedirect.com/science/article/pii/S1521661615001576>.
• Kamdar, K et al. “Genetic and Metabolic Signals during Acute Enteric Bacterial Infection Alter the
Microbiota and Drive Progression to Chronic Inflammatory Disease.” Cell Host & Microbe 19.1 (2016) :
21-31.
<http://www.cell.com/cell-host-microbe/pdfExtended/S1931-3128(15)00498-9>.
• McLean, Mairi et al. “Does the Microbiota Play a Role in the Pathogenesis of Autoimmune Diseases?” Gut
64.2 (2014) : gutjnl–2014–308514. <
http://gut.bmj.com/content/early/2014/11/28/gutjnl-2014-308514>.
• Waldor, Matthew et al. “Where next for Microbiome Research?” PLoS Biology 13.1 (2015) : e1002050.
<http://journals.plos.org/plosbiology/article?id=10.1371/journal.pbio.1002050>.
• Wu, Hao, Valentina Tremaroli, and Fredrik Bäckhed. “Linking Microbiota to Human Diseases: A Systems
Biology Perspective.” Trends in Endocrinology & Metabolism 26.12 (2015) : 758-770.
<http://www.cell.com/trends/endocrinology-metabolism/abstract/S1043-2760(15)00194-0>.
Autoimmunity and the Microbiome
@DrBonnie360

30. The Microbiome and Inflammatory Bowel Disease
• De Souza, Heitor, and Claudio Fiocchi. “Immunopathogenesis of IBD: Current State of the
Art.” Nature Reviews Gastroenterology & Hepatology (2015) : 13-27 <
http://www.ncbi.nlm.nih.gov/pubmed/26627550>.
• Sartor, R. Balfour. “The Intestinal Microbiota in Inflammatory Bowel Disease.” Nestle Nutrition
Institute Workshop Series 79 (2014) : 29–39.
<https://uncch.pure.elsevier.com/en/publications/the-intestinal-microbiota-in-inflammatory-
bowel-diseases>.
• Sartor, RB, and SK Mazmanian. “Intestinal Microbes in Inflammatory Bowel Diseases.” The
American Journal of Gastroenterology … (2012) : 15-21. <
http://www.nature.com/ajgsup/journal/v1/n1/abs/ajgsup20124a.html>.
• Wlodarska, Marta, Aleksandar Kostic, and Ramnik Xavier. “An Integrative View of Microbiome-
Host Interactions in Inflammatory Bowel Diseases.” Cell Host & Microbe 17.5 (2015) : 577-591.
<http://www.cell.com/cell-host-microbe/abstract/S1931-3128(15)00166-3>.
@DrBonnie360

31. The Microbiome and Periodontal Disease
• Costalonga, M, and MC Herzberg. “The Oral Microbiome and the Immunobiology of Periodontal
Disease and Caries.” Immunology Letters 162.2 (2014) : 22-38.
<http://www.sciencedirect.com/science/article/pii/S0165247814001874>.
Rheumatoid Arthritis
Systemic Lupus Erythematosus
• Scher, JU, and SB Abramson. “The Microbiome and Rheumatoid Arthritis.” Nature Reviews
Rheumatology 7 (2011) : 569-578. <
http://www.nature.com/nrrheum/journal/v7/n10/abs/nrrheum.2011.121.html>.
• Mu, Q, H Zhang, and XM Luo. “SLE: Another Autoimmune Disorder Influenced by Microbes and
Diet?” Frontiers in Immunology (2015) : n. pag. <
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC4663251/>.
@DrBonnie360

32. Prevention – Diet, Food, & Nutrition
• Tilg, H, and AR Moschen. “Food, Immunity, and the Microbiome.” Gastroenterology 148.6
(2015) : 1107-1119. <
http://www.sciencedirect.com/science/article/pii/S0016508515000128>.
• Zeevi et al. “Personalized Nutrition by Prediction of Glycemic Responses.” Cell. 163.5 (2015) :
1079-1094. <http://www.cell.com/abstract/S0092-8674(15)01481-6>.
• Zmora, N et al. “Taking It Personally: Personalized Utilization of the Human Microbiome in
Health and Disease.” Cell Host & Microbe 19.1 (2016) : 12-20.
<http://www.cell.com/cell-host-microbe/pdf/S1931-3128(15)00508-9.pdf>.
Prevention – Prebiotics & Probiotics
• Hemarajata, P, and J Versalovic. “Effects of Probiotics on Gut Microbiota: Mechanisms of
Intestinal Immunomodulation and Neuromodulation.” Therapeutic Advances
in Gastroenterology. (2012) : 39-51.
<http://tag.sagepub.com/content/early/2012/09/24/1756283X12459294.abstract>.
• Petschow, B et al. “Probiotics, Prebiotics, and the Host Microbiome: The Science of
Translation.” Annals of the New York Academy of Sciences (2013) : 1-17. <
http://onlinelibrary.wiley.com/doi/10.1111/nyas.12303/full>. @DrBonnie360

33. • 2008 = Human Microbiome Project. hlp://hmpdacc.org/overview/about.php
• 2012 = ScienKfic American, explore the human microbiome (interacKve) hlp://www.scienKficamerican.com/arKcle/microbiome-graphic-
explore-human-microbiome/
• 2015 = American Museum of Natural History
• exhibit: The Secret World Inside You hlp://www.amnh.org/calendar/the-secret-world-inside-you
• info: Meet your Microbiome hlp://www.amnh.org/explore/science-topics/health-and-our-microbiome/meet-your-microbiome
• 2016 = hlps://www.whitehouse.gov/the-press-office/2016/05/12/fact-sheet-announcing-naKonal-microbiome-iniKaKve